Economic analysis of the production and recovery of green fluorescent protein using ATPS-based bioprocesses

Green fluorescent protein (GFP) is a useful biomolecule in biotechnology; however, its price makes widespread application prohibitive. To overcome this issue, recently, the use of aqueous two-phase systems (ATPS) for GFP purification was proposed as an alternative platform to reduce processing costs. Aligned with goal, study performed bioprocess modelling coupled economic analysis using software Biosolve evaluate potential and commercial applicability ATPS purification. This work analysed collection fourteen discriminate through production costs while also incorporating concept product purity into calculations. The two best (a PEG-based ionic liquid (IL)-based ATPS) were placed full at different scale models (1 100 L) elucidate viability applying large scale. Although results showed that exhibit lowest (between USD 3.5x103.g?1 1 L 0.33x103.g?1 L), further developments, inclusion granting higher desired development simpler bioprocesses. Therefore, third approach work, sensitivity determine impact varying model parameters (recovery yield, material discount titre), circumstances under which IL-based system can traditional ATPS. indicate cost-effectiveness improve titre (although it affect all studied), increase from 1.33 3.8 g/L enough be less expensive than scales. demonstrates greatly manufacturing costs, potentially help popularize new applications proteins are currently mostly restricted research kits due their high prices.